|Students learn how to load and program a thermocycler which heats and cools DNA, combining them with primers, to help isolate sequences of interest for further examination.|
All of this is done in the molecular biology lab.
Enter the Lab...
At first glance, it is filled with a dizzying array of high-tech equipment, spinning, shaking, freezing, heating, pressurizing, electrifying, and illuminating test samples. The whirling of servos and the blinking of lights, along with touch screens and and the jet-like sound of spinning centrifuges might invoke feelings of a science fiction movie. It can seem intimidating, but if you have a good teacher, virtually anyone can learn to use these tools, and they can do so in a single day.
|KMUTT senior students and Dr. Dujduan Waraho look at the results from one of the electrophoresis gel runs. By looking at the bands produced during the runs, students were able to learn more about their blood types.|
The seniors themselves are studying chemical engineering, so for them, this initial workshop was as much a lesson to them, as their finished senior project will be to local high school students.
We were lucky enough to be invited to take part in the workshop as well. We have followed KMUTT's interest in teaching others about biotech for some time.
|Dr. Dujduan Waraho prepares an electrophoresis gel before running our samples.|
Throughout the 2-day workshop, we learned not only what we could do next time for more reliable and accurate results, but also how to make the entire process more efficient and less time consuming.
Not As Hard As It Looks...
We all already knew our blood types, but the point of the workshop was to give us an introduction into using the tools found in a modern molecular biology lab. These are the same tools being used to conduct cutting edge genetic research on humans and other organisms, as well as the same tools used to perform genetic engineering, gene therapy, forensics, and disease detection.
|Loading electrophoresis wells.|
From here, the small team of KMUTT students will refine the protocol to make it easier and quicker for their own workshop they plan on running with high school students.
For us, we plan on bringing the knowledge we acquired back to Thailand's first community lab, F.Lab, and use it to help inform the design of our community space, and to help organize future workshops to spread an interest in biotechology among regular people. F.Lab seeks to give people the tools they need to become participants and producers rather than mere spectators and consumers in this increasingly important field of technology.
F.Lab's first project will be DNA barcoding important organic crops for farmers in Southeast Asia. It will be the beginning of a more accurate and more decentralized means of quality control. When you buy rice from a Thai farmer who has DNA barcoded their rice, you will know exactly what you are putting on your plate. Combined with an opensource auditing system being developed by other F.Lab members, Thailand's organic scene will become more transparent and dependable than ever.
Working in a real lab also gives us insight into various kinds of lab equipment. F.Lab will also feature opensource DIYbio lab equipment already being developed (and in fact, already brought to and tested during the workshop at KMUTT). Seeing and working with industry equipment helps better inform the design of their opensource alternatives.
|F.lab's opensource 3D printed mini-centrifuge at KMUTT's molecular biology lab. It was even used during a 'quick spin' while following the protocols. Working with real professionals in a real lab gives DIYbio enthusiasts inspiration to take their designs and ideas to the next level.|
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